Analytical methods for STEC/VTEC detection and other categories of diarrheagenic E. coli in foods (meat, dairy products and vegetables) and clinical samples. Detection of STEC/VTEC O104:H4, O157:H7 and others serotypes by conventional and real-time PCR.
E. coli vaccines.
Intimin typing by PCR and DNA sequencing of eae gene of the LEE pathogenicity island.
Molecular typing by PFGE and MLST.
Pathogenesis, diagnostic and molecular typing of verocytotoxigenic Escherichia coli (VTEC/STEC) O104:H4, O157:H7, O26:H11, O103:H2, O111:H8, O121:H19, O145:H- and other serotypes. Detection in clinical samples and food.
Pathogenesis, epidemiology and microbiological diagnostic of E. coli strains that cause infections in humans and animals. STEC/VTEC, ETEC, EPEC, EIEC, EAggEC, AEEC, AIEC, ExPEC
Serotypes, virulence genes and molecular typing of E.coli producing extended-spectrum beta-lactamases (ESBL). Characterization of the intercontinental emergence clone O25:H4-ST131 producing CTX-M-15.
Serotypes, virulence genes and molecular typing of extraintestinal pathogenic Escherichia coli (ExPEC).
Spanish National Reference Laboratory for the Detection of E. coli in Food (AESAN, Ministerio de Sanidad).