Nanoengineered delivery of RNAimpact on brain diseases and COVID-19 vaccination

  1. López Borrajo, Mireya
Supervised by:
  1. María José Alonso Fernández Director

Defence university: Universidade de Santiago de Compostela

Fecha de defensa: 22 December 2023

Committee:
  1. Bruno Filipe Carmelino Cardoso Sarmento Chair
  2. María Dolores Torres López Secretary
  3. Maria Jesús Vicent Docon Committee member
Department:
  1. Department of Pharmacology, Pharmacy and Pharmaceutical Technology

Type: Thesis

Teseo: 830208 DIALNET lock_openMINERVA editor

Abstract

Genetic therapies show great potential for treating various diseases through modulation of the expression of specific genes. Their effective delivery is crucial, particularly for challenging conditions like CNS diseases and SARS-CoV-2 vaccines. Here, we used nanotechnology as a fundamental tool for RNA delivery. First, we engineered an ionizable nanoemulsion designed for brain diffusion and delivery of RNA. Second, we explored nanocarriers loaded with siRNA for brain delivery, exploring their performance following alternative administration routes. Finally, we encapsulated mRNAs derived from SARS-CoV-2 within nanoemulsions and polymeric nanocapsules, achieving significant cellular immune responses.