Selective recognition of threeway DNA junctions with designed peptidomimetics
- Alcalde Ordóñez, Ana
- M. Eugenio Vázquez Doktorvater
- Miguel Vázquez López Doktorvater
Universität der Verteidigung: Universidade de Santiago de Compostela
Fecha de defensa: 13 von Juli von 2023
- Luca Salassa Präsident/in
- Beatriz Pelaz García Sekretärin
- Michael John Hannon Vocal
Art: Dissertation
Zusammenfassung
Non-canonical DNA structures, such as three-way DNA junctions (3WJs), formed during DNA replication, have emerged as promising alternative DNA targets for the development of new drugs with attractive therapeutic properties. In this context, supramolecular helicates have shown high selectivity and binding affinity for 3WJs, but their use in this field is hampered by the lack of synthetic versatility and a general strategy to their enantioselective synthesis. In this thesis, we describe the stereoselective self-assembly of helicates derived from peptide ligands equipped with 2,2’-bipyridine coordinating residues, as well as the implementation of a new strategy that relies on the cooperative 3WJ-binding mechanism of natural proteins, to yield new systems with selective 3WJs recognition properties.