Designed metallopeptides as tools in chemical biology

  1. Ghofrane Barka
Dirixida por:
  1. M. Eugenio Vázquez Director
  2. Miguel Vázquez López Director

Universidade de defensa: Universidade de Santiago de Compostela

Ano de defensa: 2017

  1. Ignacio Alfonso Rodríguez Presidente/a
  2. Elena Pazos Secretario/a
  3. Fernando José López García Vogal
  1. Departamento de Química Orgánica

Tipo: Tese


A series of Ru(II) metallopeptides featuring the dipyrido[3,2-a:2',3'-c]phenazine ligand (dppz) have been synthesized through solid-phase peptide synthesis (SPPS) methods. Spectroscopic studies indicate that functionalization with an oligoarginine basic tail causes a drastic change in the binding mode and a great increase of the affinity of these metallopeptides for well-matched B-DNA oligonucleotides over mismacthed DNAs. In addition, the spectroscopic data suggest that both the oligoarginine functionalization and the nature of the ancillary ligands do not cause an increase of the selectivity of these metallopeptides for a particular type of mismatched DNA oligonucleotide. Finally, fluorescence microscopy studies indicate that the oligoarginine functionalization causes the efficient internalization of the metallopeptides into Vero cells, causing apoptotic cell death after a few hours.