Identification of New Molecular Biomarkers with High Diagnostic Accuracy to Detect Untreated Periodontitis and its Response to Treatment

  1. Blanco Pintos, Triana
Supervised by:
  1. Inmaculada Tomás Carmona Director
  2. Manuela Alonso Sampedro Director

Defence university: Universidade de Santiago de Compostela

Fecha de defensa: 24 October 2023

Committee:
  1. Luis Miguel Moutinho da Silva Monteiro Chair
  2. Francisco Gude Sampedro Secretary
  3. Aurea Simón-Soro Committee member
Department:
  1. Department of Surgery and Medical-Surgical Specialities

Type: Thesis

Teseo: 819030 DIALNET lock_openMINERVA editor

Abstract

The objective of this thesis was to discover new biomarkers present in the oral fluids for discriminating with high accuracy the presence of periodontitis. Firstly, we conducted a systematic review and meta-analysis, in which we demonstrated that combinations of two biomarkers in gingival crevicular fluid and saliva have a high accuracy in the diagnosis of periodontitis, which does not increase with the incorporation of additional biomarkers. Subsequently, using the SWATH-MS proteomic technique, we quantified 250 proteins in gingival crevicular fluid and 375 in saliva from periodontally healthy subjects, patients with untreated periodontitis, and treated periodontitis. The predictive modelling of these molecules allowed the discovery of new proteins with high accuracy in detecting both untreated and treated periodontitis.