Flow cytometric study of phagocytic and oxidative burst activities of turbot head-kidney leucocytes in response to Aeromonas salmonicida subspecies salmonicida and Tenacibacterium maritimum
- 1 Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Editorial: Research Singpost
ISBN: 81-308-0022-5
Ano de publicación: 2006
Páxinas: 133-143
Tipo: Capítulo de libro
Resumo
The present work describes flow cytometry based methods for the measurement of phagocytic activity and respiratory burst of head-kidney leucocytes of turbot (Scophthalmus maximus) and to characterize the leucocytes subpopulations. Optimal conditions leucocyte collection and analysis as well as for study of phagocytic and respiratory burst activities of turbot head-kidney leucocytes in response to Aeromonas salmonicida subspecies salmonicida and Tenacibaculum maritimum were determined. The methods developed are rapid and easy to perform, requires small number of leucocytes, are suitable for use in relatively large groups of animals and provide quantitative results. Phagocytosis assay shown that head-kidney turbot leucocytes display a phagocytic ability of 29% and 46% using as target particle formalin-inactivated A. salmonicida and T. maritimum cells, respectively. A clear increase of active turbot phagocyte population was detected after stimulation with the soluble activator PMA or with formalin-inactivated A. salmonicida and T. maritimum cells.