Photobacterium damselae subsp. damselaeanalysis of the genetic diversity and characterization of a conserved two-component system regulating virulence
- Terceti, Mateus de Souza
- Carlos Rodríguez Osorio Director
Universidade de defensa: Universidade de Santiago de Compostela
Fecha de defensa: 13 de decembro de 2018
- Jesús Manuel Aparicio Fernández Presidente/a
- Trinidad de Miguel Bouzas Secretaria
- Sandra Manuela Rodrigues Sousa Vogal
Tipo: Tese
Resumo
Photobacterium damselae subsp. damselae is an emerging pathogen of marine aquaculture. By combining phenotypic and genotypic data, and comparative genomics analyses, we here show that this pathogen exhibits a high genetic diversity and that outbreaks in fish are caused by multiclonal populations. We identified and characterized the RstAB two-component signal transduction system, a major positive regulator of virulence and of many aspects of cell physiology in this bacterium. The RstAB regulon comprises the major cytotoxins Dly, PhlyP and PhlyC, as well as hitherto unreported proteins secreted by the type two secretion system (T2SS), that may constitute novel virulence factors in this pathogen.