In vitro bactericidal activity of equine platelet concentrates, platelet poor plasma, and plasma against methicillin-resistant Staphylococcus aureus

  1. ME Álvarez
  2. C López
  3. CE Giraldo
  4. I Samudio
  5. JU Carmona
Revista:
Archivos de Medicina Veterinaria

ISSN: 0301-732X,  0717-6201

Año de publicación: 2011

Volumen: 43

Número: 2

Páginas: 155-161

Tipo: Artículo

DOI: 10.4067/S0301-732X2011000200008 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Archivos de Medicina Veterinaria

Resumen

Los objetivos del estudio fueron: 1) evaluar el efecto antibacteriano de concentrados de plaquetas equinas (ePC) (activados o no con gluconato de calcio (CG)) frente a Staphylococcus aureus meticilino-resistente (MRSA) y 2) comparar su efecto antibacteriano contra plasma pobre en plaquetas (PPP) (activado con CG -PPP/GC-) y plasma (P). Los productos sanguíneos fueron divididos en 4 grupos (ePC, ePC/CG, PPP/CG y P), más un grupo control positivo (PCG) y otro control negativo. Los grupos se mezclaron con caldo Mueller-Hinton y MRSA. Las muestras fueron incubadas durante 1, 4, 8, 12 y 24 horas y se contaron las unidades formadoras de colonias. El crecimiento de las bacterias fue significativamente (P = 0,01) inhibido por el ePC, ePC/CG, PPP/CG y P en comparación con el PCG durante las primeras 12 h. Sólo a las 24 horas hubo una diferencia estadísticamente significativa (P = 0.01) y se observó un efecto antibacteriano para el ePC, ePC/CG y PPP/CG en comparación con el PCG y P. Los ePCs y PPP equinos mostraron el mejor efecto antibacteriano in vitro contra el MRSA.

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Fuente de los datos: Dialnet