Estudio clínico de evolución y pronóstico de la leucoplasia oral y su relación con la expresión de la anhidrasa carbónica IX

Abstract

A wide variety of lesions exists in the field of oral pathology, some of which deserve more attention than others. Leukoplakia or erythroplasia are considered by WHO as potentially malignant or precancerous lesions, because of they have a high probability of malignant transformation. The standard treatments for these injuries come from a careful observation to complete resection. Factors that are influencing more in the malignant transformation of these lesions are not known yet with certainty. These factors include clinical type, site, grade of epithelial dysplasia, smoking, and alcohol intake. Epithelial dysplasia is a factor that is taken into account at the time of treatment or other. The valuation of this factor is difficult and subjective, with a remarkable variability among different observers. CA-IX is expressed primarily in tumor cells, hence it´s considered as a high specificity hypoxia marker in tumors, with great value both prognosis and diagnosis in tumor therapy. The aim of this work is to determine the expression of CA-IX in oral dysplastic precancerous lesions with a diagnostic and prognostic purpose in the evolution of these lesions. This requires an analysis of the expression of CA-IX in blood and tissue, in patients with oral lesions that have different degrees of dysplasia. Determination of CA-IX in tissue samples will be performed by immunohistochemistry, while the sample of peripheral blood and tissue samples RNA of CA-IX will be determined by PCR probe.