Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy

  1. Pena-Rodríguez, Eloy 4
  2. García-Berrocoso, Teresa 56
  3. Vázquez Fernández, Ezequiel 1
  4. Otero-Espinar, Francisco J. 123
  5. Abian, Joaquin 56
  6. Fernández-Campos, Francisco 4
  1. 1 Pharmacology, Pharmacy and Pharmaceutical Technology Department, Faculty of Pharmacy, University of Santiago de Compostela (USC), Santiago de Compostela, Spain
  2. 2 Parqueasil Group, Health Research Institute of Santiago de Compostela (FIDIS), Santiago de Compostela, Spain
  3. 3 Institute of Materials (iMATUS), University of Santiago de Compostela (USC), Santiago de Compostela, Spain
  4. 4 Laboratory Reig Jofre, R&D Department, 08970, Sant Joan Despí, Barcelona, Spain
  5. 5 Biological and Environmental Proteomics, Institut d'Investigacions Biomèdiques de Barcelona, Consejo Superior de Investigaciones Científicas (IIBB-CSIC), Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
  6. 6 Laboratorio de Proteómica CSIC/Universitat Autònoma de Barcelona (UAB), IIBB-CSIC, Barcelona, Spain
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Journal:
International Journal of Pharmaceutics

ISSN: 0378-5173

Year of publication: 2023

Volume: 636

Pages: 122808

Type: Article

DOI: 10.1016/J.IJPHARM.2023.122808 GOOGLE SCHOLAR lock_openOpen access editor

More publications in: International Journal of Pharmaceutics

Abstract

Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 μm) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions.

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