Isolation and typing methods for the epidemiologic investigation of thermotolerant campylobacters
- Jorge Barros Velázquez 1
- Ana Jiménez
- Tomás González Villa 2
- 1 Food Technology Section, Higher Technological School, University of Santiago, Lugo, Spain
- 2 Department of Microbiology, Faculty of Pharmacy, University of Santiago, Santiago de Compostela, Spain
ISSN: 1618-1905
Ano de publicación: 1999
Volume: 2
Número: 4
Páxinas: 217-226
Tipo: Artigo
Outras publicacións en: International microbiology: official journal of the Spanish Society for Microbiology
Resumo
Thermotolerant campylobacters, C. jejuni, C. coli, C. lari and C. upsaliensis, are spiral bacteria involved in human enteric disease. The prevalence of these emerging pathogens, mainly C. jejuni and to a lesser extent C. coli, as etiologic agents of enteric disease in industrialized countries has increased over the last decade. The isolation and culture of these microorganisms is tedious and timeconsuming mainly due to their complex nutritional and environmental requirements. This review discusses the techniques and methods developed for the selective isolation of thermotolerant campylobacters from food, environmental and clinical samples. Additionally, both traditional and newer molecular biology techniques applied to this group of thermophilic organisms for typing and taxonomic purposes are summarized.